A ratiometric fluorescence probe for selective and sensitive detection of leucine aminopeptidase in lysosome
Di Yuan, Ziwei Xu, Bingling Zhang, Xiong Yin, Jiqing Ye, Xiao‐Le Zhou, Leyu Wang
Abstract
We designed a novel ratiometric fluorescent probe P1-Leu with a donor-acceptor-donor fluorophore for the detection of leucine aminopeptidase in lysosomes. P1-Leu exhibits a lower detection limit than the ratiometric donor-π-acceptor probe, due to the low ratiometric background. Besides, P1-Leu has good lysosome-targeting ability and realizes the distinction of LAP levels in different cells.
Topics & Concepts
FluorophoreLysosomeFluorescenceAminopeptidaseAcceptorChemistryLeucineFluoresceinDetection limitBiochemistryEnzymeAmino acidChromatographyPhysicsQuantum mechanicsCondensed matter physicsMolecular Sensors and Ion DetectionAdvanced biosensing and bioanalysis techniquesCarbon and Quantum Dots Applications