Total Phenolic Contents, DPPH Radical Scavenging and β-Carotene Bleaching Activities of Aqueous Extract from Ammoides atlantica
Karima Loucif, Hassiba Benabdallah, Fatima Benchikh, Soulaf Mehlous, Chawki Ben Souici, Smain Amira
Abstract
Phytotherapy has known a great evolution all the world and some medicinal plants are important remedies of some diseases. Ammoides atlantica is one of the medicinal plants used in folk medicine. This study aims to estimate the total phenolics and flavonoids contents then to investigate both in vitro antioxidant activity models of aqueous extract (AqE) from Ammoides atlantica. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 Method. The in vitro antioxidant property was assessed by DPPH-scavenging radicals and lipid peroxidation assays. The results revealed that Ammoides atlantica aqueous extract presented a high total phenolic and flavonoid contents with values of 85.56±4.71 µg GAE (gallic acid equivalent)/mg and 40.55±4.09 µg QE (quercitin equivalent)/mg dry extract, respectively. This extract shows a good DPPH radical scavenging and β-carotene bleaching activities with an IC50 of 107.48±5.9 µg/mL and 130.17±5.52 µg/mL, respectively. This study indicates that the aqueous extract from Ammoides atlantica has potent antioxidant effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants drugs.
 Keywords: Ammoides atlantica, aqueous extract, antioxidant activity, phenolic compounds.