Role of Berberine as a Potential Efflux Pump Inhibitor against MdfA from Escherichia coli: <i>In Vitro</i> and <i>In Silico</i> Studies
Ying Li, Xizhen Ge
Abstract
. We demonstrated that low levels of BBR significantly increased intracellular ciprofloxacin concentrations and restored antibiotic susceptibility of the reporter strain. Molecular dynamics simulations revealed the effect of BBR on the conformational transition of MdfA. Our data suggested that driving forces for MdfA's conformational transition were affected by BBR and provided evidence for BBR's extended application as an effective inhibitor of MdfA.
Topics & Concepts
In silicoEffluxEscherichia coliBerberineIn vitroBiologyChemistryComputational biologyGeneticsPharmacologyGeneDrug Transport and Resistance MechanismsBerberine and alkaloids researchAntibiotic Resistance in Bacteria