Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a <i>trans</i>-cleavage with MnO<sub>2</sub> nanosheets
Renpeng Xia, Nan Ouyang, Tingting Wang, Yuan Zhuang, Peng Miao
Abstract
-cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.
Topics & Concepts
GlutathioneCRISPRElectrochemistryChemistryCleavage (geology)Combinatorial chemistryOxidative cleavageMaterials scienceBiochemistryElectrodeCatalysisEnzymePhysical chemistryGeneComposite materialFracture (geology)Advanced biosensing and bioanalysis techniquesAdvanced Nanomaterials in CatalysisCRISPR and Genetic Engineering