ClpX Is Essential and Activated by Single-Strand DNA Binding Protein in Mycobacteria
Jemila C. Kester, Olga Kandror, Tatos Akopian, Michael R. Chase, Junhao Zhu, Eric J. Rubin, Alfred L. Goldberg, Sarah M. Fortune
Abstract
, imposes a major global health burden, surpassing HIV and malaria in annual deaths. The ClpP1P2 proteolytic complex and its cofactor ClpX are attractive drug targets, but their precise cellular functions are unclear. This work confirms ClpX's essentiality and describes a novel interaction between ClpX and SSB, a component of the DNA replication machinery. Further, we demonstrate that a loss of ClpX is sufficient to interrupt DNA replication, suggesting that the ClpX-SSB complex may play a role in DNA replication in mycobacteria.
Topics & Concepts
BiologyMycobacterium tuberculosisMalariaTuberculosisBacterial proteinDNAHuman immunodeficiency virus (HIV)Plasma protein bindingDrug targetDNA-binding proteinComputational biologyMicrobiologyVirologyGeneticsImmunologyCell biologyTranscription factorBacteriaBiochemistryGenePathologyMedicineTuberculosis Research and EpidemiologyMycobacterium research and diagnosisAntibiotic Resistance in Bacteria