In situ experimental exposure of fruit-bearing shoots of apple trees to 13CO2 and construction of a dynamic transfer model of carbon
Shogo Imada, Takashi Tani, Yasuhiro Tako, Yuki Moriya, Shun’ichi Hisamatsu
Abstract
Evaluating the transfer and metabolism of carbon (C) in apple fruit is key to estimating the potential accumulation of atmospheric 14C in fruit near and around nuclear facilities. We developed a dynamic compartment model for apple fruit-bearing shoots, assuming that the shoots are a simple unit of source and sink for photoassimilates. Fruit-bearing shoots of Malus domestica “Fuji” at different fruit growth stages were exposed to 13CO2 in situ, followed by sampling at 72 h after exposure or at harvest. The 13C/(13C+12C) mole ratio in fruits, leaves, and current branch were measured to construct a five-compartment model of 13C (fruit, each fast and slow component of leaves, and current branch). The C inventories in the compartments were presented in accordance with the measured growth curves of C in the organs. The model simulated the 13C dynamics in plant tissues well. Simulation results of photoassimilate distribution using the model indicated that the retention of photoassimilated C at the harvest depended on the growth rate of C in the organs at the exposure.