Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus
Subhransu Sekhar Behera, Amrito Ghosh Majumdar, Himansu Gullani, Arka Lodh Roy, Aruna Kumar Dash, Pratyush Kumar Behera, Zahra Parwez, Seemon Giri, Priti S. Mohanty, L. Bryan Ray
Abstract
The green synthesis of silver-chitosan nanocomposite (AgCs) is a promising avenue in nanobiotechnology, offering eco-friendly production methods and multifunctional properties. This study elucidates the green synthesis of AgNp through a sustainable approach using Streptomyces griseoincarnatus RB7AG. The synthesized AgNp was then combined with chitosan to form a silver-chitosan nanocomposite (AgCs nanocomposite) and investigated its properties employing UV–visible spectroscopy, FTIR techniques, and dynamic light scattering (DLS). The UV–visible spectroscopy confirms the successful formation of the nanocomposite, while DLS elucidates its size distribution and stability. Moreover, the antibiofilm capabilities of AgCs are evaluated against bacterial biofilms comprising Staphylococcus aureus and Escherichia coli . Remarkably, the nanocomposite demonstrates the most potent antibiofilm activity, significantly reducing biofilm metabolic activity. This observation is further corroborated by fluorescence imaging, providing visual confirmation of the reduction in biofilm formation. These findings underscore the potential of AgCs as an effective antimicrobial agent against bacterial biofilms, with implications for combating biofilm-associated infections. Furthermore, the nanocomposite was found to be non-toxic to the HEK293 cell lines, demonstrating its effectiveness in further applications. • Silver nanoparticles synthesized using Streptomyces griseincarnatus RB7AG, is a green and eco-friendly approach. • Silver chitosan nanocomposite developed, enhanced the antimicrobial properties through a synergistic effect. • The nanocomposite demonstrated effective inhibition of bacterial biofilms of E. coli and S. aureus. • Nanocomposite was non-toxic to HEK-293 cell lines.