Melatonin reduces cadmium accumulation through cell wall fraction fixation capacity in cotton seedlings
Muhammad Uzair, Yi Zhang, Abid Ali, Huazu Li, Yifei Han, Yue Sun, Jielin Wang, Wanru Wang, Samrana Samrana, Sarzamin Khan, Azizullah Azizullah, Iqbal Hussain, Imran Ali, Tianlun Zhao, Shuijin Zhu
Abstract
Melatonin (MT) participates in plants' responses in plants’ response to cadmium (Cd) tolerance, although its work model remains elusive. Here, the function of MT in adjusting Cd accumulation in Gossypium hirsutum (CCRI12) was investigated. In this research we observed that exogenous 50 μM MT significantly reduced the Cd accumulation under 100 μМ Cd stress for 7 days of treatment in cotton seedlings. Cd markedly induced the ROS (H2O2, O2-) accumulation and elevated the expression of transporters for Cd uptake and translocation. After application of MT reduced ROS and MDA accumulation in response to high induction of antioxidant defense system; the genes encoding superoxide dismutase (GhSOD), catalase (GhCAT), and peroxidase (GhPOD), ascorbate peroxidase (GhAPX), and glutathione S-transferase (GhGST) were significantly upregulated accompanied by their level of content. Furthermore, MT down-regulated transporters for Cd uptake include Natural Resistance-Associated Macrophage Protein 1 (GhNRAMP3), Zinc/Iron-regulated transporter-like protein (GhZIP1/4/6), Heavy Metal ATPase gene (GhHMA2), and ATP-binding Cassette sub family C (GhABCC3/2). Exogenous application of MT raised the hemicelluloses, cellulose and pectin content accompanied by their expression levels, xyloglucan endotransglucosylase/hydrolase (GhXTH28/6/9/B), cellulose synthase like (GhCSLG1/2) and COBRA-like (GhCOBL4), pectin acetylesterases (GhPAE8/7), pectin methylesterases (GhPME31) which in turn increased the cell wall's binding capacity to Cd.