AP-4 regulates neuronal lysosome composition, function, and transport via regulating export of critical lysosome receptor proteins at the trans-Golgi network
Piyali Majumder, Daisy Edmison, Catherine Rodger, Sruchi Patel, Evan Reid, Swetha Gowrishankar
Abstract
Using CRISPRi-based depletion in human iPSC-derived neurons we demonstrate that AP-4 adaptor protein complex regulates neuronal lysosome composition and function via regulating sorting of critical lysosomal protein receptors including Sortilin 1 to the organelle. AP-4 also regulates axonal endo-lysosome abundance in these human neurons.
Topics & Concepts
LysosomeBiologyCell biologyGolgi apparatusFunction (biology)ReceptorBiochemistryEnzymeEndoplasmic reticulumCellular transport and secretionAutophagy in Disease and TherapyCalcium signaling and nucleotide metabolism