Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer
Yin Peng, Xiaojing Zhang, Huijuan Lin, Shiqi Deng, Ying� Qin, Jieqiong He, Fan Hu, Xiaohui Zhu, Xianling Feng, Jian Wang, Yanjie Wei, Xinmin Fan, Huan Lin, Hassan Ashktorab, Duane T. Smoot, Yansi Lv, Song Li, Stephen J. Meltzer, Zhe Jin
Abstract
, suggesting the potential application of miRNA-150 inhibitors in GC therapy. The expression of genes downstream of Hh and Wnt signaling was also reduced in tumors treated with miRNA-150 inhibitors. Notably, anti-SUFU siRNAs rescued the inhibitory effects of miRNA-150 inhibitors on Wnt signaling, Hh activation, EMT, cell proliferation, cell migration, and colony formation. Taken together, these findings indicate that miRNA-150 is oncogenic and promotes GC cell proliferation, migration, and EMT by activating Wnt and Hh signaling via the suppression of SUFU expression.