Teaching indicators to unravel the kinetic features of host–guest inclusion complexes
Amrutha Prabodh, Stephan Sinn, Laura Grimm, Zsombor Miskolczy, Mónika Megyesi, László Biczók, Stefan Bräse, Frank Biedermann
Abstract
Both thermodynamic and kinetic insights are needed for a proper analysis of association and dissociation processes of host-guest interactions. However, kinetic descriptions of supramolecular systems are scarce in the literature because suitable experimental protocols are lacking. We introduce here three time-resolved methods that allow for convenient determination of kinetic rate constants of spectroscopically silent or even insoluble guests with the macrocyclic cucurbit[n]uril family and human serum albumin (HSA) protein as representative hosts.
Topics & Concepts
Supramolecular chemistryKinetic energyHost (biology)Inclusion (mineral)ChemistryLigand (biochemistry)Kinetic controlMechanism (biology)Combinatorial chemistryCrystallographyOrganic chemistryBiologyMineralogyCatalysisBiochemistryPhysicsCrystal structureQuantum mechanicsEcologyReceptorSupramolecular Chemistry and ComplexesCrystallography and molecular interactionsProtein Structure and Dynamics