One-Step Rapid and Sensitive ASFV p30 Antibody Detection via Nanoplasmonic Biosensors
Ya Zhao, Rui Li, Changjie Lv, Yuanfeng Zhang, Hanlin Zhou, Xiaohan Xia, Shiman Yu, Yongqi Wang, Liping Huang, Qiang Zhang, Gang L. Liu, Meilin Jin
Abstract
African swine fever (ASF) is a serious transnational disease caused by the African swine fever virus (ASFV), which is highly contagious in wild boars and domestic pigs. There is currently no available vaccine for ASF; therefore, development efforts are a key priority as ASFV continues to evolve and diversify. The ASF antibody rapid detection platform comprising the nanoplasmonic biosensor with extraordinary optical transmission effect can greatly reduce the detection time and improve detection flux while maintaining detection sensitivity and specificity. The one-step sample addition can effectively avoid cross contamination of samples in the detection process. The detection method provides a solution for the rapid and accurate real-time monitoring of ASF in pig farms.