Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris
X. R. Zhang, Tong Ma, Yuchen Wang, Shan Hu, Y. Yang
Abstract
Candida auris is a highly lethal, multidrug-resistant, invasive fungus. However, conventional methods of C. auris identification are time-consuming and laborious and have low sensitivity and high error rates. In this study, a new molecular diagnostic method based on recombinase-aided amplification combined with lateral flow strips (RAA-LFS) was developed, and accurate results could be obtained by catalyzing the reaction at body temperature for 15 min. This method can be used for rapid clinical detection of C. auris, consequently saving valuable treatment time for patients.
Topics & Concepts
Candida aurisMultiple drug resistanceIdentification (biology)VisualizationBiologyComputational biologyComputer scienceDrug resistanceMicrobiologyAntifungalArtificial intelligenceBotanyAntifungal resistance and susceptibilityFungal Infections and StudiesPlant Pathogens and Fungal Diseases