Single-cell RNA sequencing and spatial transcriptomics reveal unique subpopulations of infiltrating macrophages and dendritic cells following AKI
Arin L. Melkonian, Matthew D. Cheung, Elise N. Erman, Kyle H. Moore, Jeremie M. Lever, Yanlin Jiang, Zhengqin Yang, Brittany N. Lasseigne, Anupam Agarwal, James F. George
Abstract
In this work, we fully characterized both single cell and spatial transcriptomes of kidney infiltrating macrophages (KIMs) and kidney dendritic cells (KDCs) following bilateral ischemia reperfusion injury. We also discovered distinct markers that differentiate KIMs from one another and kidney resident macrophages (KRMs). Finally, we show evidence suggesting that KIMs may reprogram and express genes previously limited to KRMs by day 28 following injury resolution.
Topics & Concepts
Acute kidney injuryTranscriptomeKidneyBiologyInflammationMacrophageKidney diseasePopulationPathologyCancer researchMedicineGeneImmunologyGene expressionInternal medicineEndocrinologyGeneticsEnvironmental healthIn vitroAcute Kidney Injury ResearchChronic Kidney Disease and DiabetesDialysis and Renal Disease Management