Incoherent color digital holography with computational coherent superposition for fluorescence imaging [Invited]
Tatsuki Tahara, Takako Koujin, Atsushi Matsuda, A. Ishii, Tomoyoshi Ito, Yasuyuki Ichihashi, Ryutaro Oi
Abstract
We present color fluorescence imaging using an incoherent digital holographic technique in which holographic multiplexing of multiple wavelengths is exploited. Self-interference incoherent digital holography with a single-path in-line configuration and the computational coherent superposition scheme are adopted to obtain color holographic three-dimensional information of self-luminous objects with a monochrome image sensor and no mechanical scanning. We perform not only simultaneous color three-dimensional sensing of multiple self-luminous objects but also color fluorescence imaging of stained biological samples. Color fluorescence imaging with an improved point spread function is also demonstrated experimentally by adopting a Fresnel incoherent correlation holography system.