Skermanella pratensis sp. nov., isolated from meadow soil, and emended description of the genus Skermanella
Qiannan Guo, Zhengfu Zhou, Lijuan Zhang, Chen Zhang, Ming Chen, Bo Wang, Min Lin, Wei Wang, Wei Zhang, Xufeng Li
Abstract
A novel Gram-stain-negative, light pink-coloured, short rod-shaped, designated strain W17 T , was isolated from a meadow soil sample collected from Xinjiang, PR China. The 16S rRNA gene sequence analysis indicated that strain W17 T was related most closely to Skermanella rosea M1 T (98.72 %) and Skermanella mucosa 8-14-6 T (98.44 %). However, strain W17 T showed a low level of DNA–DNA relatedness to S. rosea M1 T (32.4±2.6 %) and S. mucosa 8-14-6 T (33.5±0.1 %). The genome size of the novel strain was 5.87 Mb and the genomic DNA G+C content was 67.27 mol%. The only respiratory quinone of strain W17 T was Q-10. Diphosphatidylglycerol, phosphatidylglycerol. phosphatidylethanolamine and phosphatidylcholine were the major polar lipids. The predominant cellular fatty acids were C 18 : 1 ω6 c and/or C 18 : 1 ω7 c (48.53 %), C 16 : 0 (20.88 %) and C 18 : 0 (14.92 %). The phylogenetic, phenotypic and chemotaxonomic data showed that strain W17 T represents a novel species of the genus Skermanella , for which the name Skermanella pratensis sp. nov. is proposed. The type strain is W17 T (=GDMCC 1.1392 T =KCTC 62434 T ).