Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells
Yanan Zhu, Dapeng Sun, Andreas Schertel, Jiying Ning, Xiaofeng Fu, Pam Pam Gwo, Alan M. Watson, Laura C. Zanetti-Domingues, Marisa L. Martin-Fernandez, Zachary Freyberg, Peijun Zhang
Abstract
The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary fibroblasts from a Leigh syndrome patient harboring a disease-causing mutation in USMG5 protein responsible for impaired mitochondrial energy production.