Production and characterization of a single-chain variable fragment antibody from a site-saturation mutagenesis library derived from the anti-Cry1A monoclonal antibody
Sa Dong, Meijing Gao, Zongyi Bo, Lingjun Guan, Xiaodan Hu, Hanxiaoya Zhang, Beibei Liu, Pan Li, Kangli He, Xianjin Liu, Cunzheng Zhang
Topics & Concepts
Saturated mutagenesisMolecular biologyChemistryMonoclonal antibodyImmunoglobulin light chainBiopanningOverlap extension polymerase chain reactionComplementarity determining regionAntibodyMutantBiologyBiochemistryPeptide sequencePolymerase chain reactionPeptide libraryGeneGeneticsInsect Resistance and GeneticsCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniques