Silencing alanine transaminase 2 in diabetic liver attenuates hyperglycemia by reducing gluconeogenesis from amino acids
Michael R. Martino, Manuel Gutiérrez‐Aguilar, Nicole K.H. Yiew, Andrew J. Lutkewitte, Jason Singer, Kyle S. McCommis, Daniel Ferguson, Kim Liss, Jun Yoshino, M. Katie Renkemeyer, Gordon I. Smith, Kevin Cho, Justin A. Fletcher, Samuel Klein, Gary J. Patti, Shawn C. Burgess, Brian N. Finck
Abstract
C-alanine into newly synthesized glucose by hepatocytes. In vivo Gpt2 knockdown or knockout in liver has no effect on glucose concentrations in lean mice, but Gpt2 suppression alleviates hyperglycemia in db/db mice. These data suggest that ALT2 plays a significant role in hepatic gluconeogenesis from amino acids in diabetes.
Topics & Concepts
GluconeogenesisAlanineAlanine transaminaseAspartate transaminaseHepatocyteTranscription factorEndocrinologyInternal medicineBiochemistryChemistryEndoplasmic reticulumAmino acidBiologyGeneMedicineMetabolismEnzymeIn vitroAlkaline phosphatasePancreatic function and diabetesMetabolism, Diabetes, and CancerEndoplasmic Reticulum Stress and Disease