Kir2.1 Interactome Mapping Uncovers PKP4 as a Modulator of the Kir2.1-Regulated Inward Rectifier Potassium Currents
Sung Soo Park, Daniela Ponce‐Balbuena, Rork Kuick, Guadalupe Guerrero‐Serna, Justin Yoon, Dattatreya Mellacheruvu, Kevin Conlon, Venkatesha Basrur, Alexey I. Nesvizhskii, José Jalife, Jean‐François Rual
Abstract
, a trafficking deficient ATS1 mutant, thus uncovering molecular mechanisms whose malfunctions may underlie ATS1 disease. Finally, using patch-clamp analysis, we validate the functional relevance of PKP4, one of our top BioID interactors, to the modulation of Kir2.1-controlled inward rectifier potassium currents. Our results validate the power of our BioID approach in identifying functionally relevant Kir2.1 interactors and underline the value of our Kir2.1 interactome as a repository for numerous novel biological hypotheses on Kir2.1 and Kir2.1-associated diseases.