Development of a colorimetric α-ketoglutarate detection assay for prolyl hydroxylase domain (PHD) proteins
Samantha J. Wong, Alison E. Ringel, William Yuan, João A. Paulo, Haejin Yoon, Mark A. Currie, Marcia C. Haigis
Abstract
values of pan-PHD inhibitors and several PHD2 inhibitors in clinical trials for chronic kidney disease (CKD)-induced anemia. Given the efficiency of this assay coupled with its multiwell format, the 2,4-DNPH α-KG assay may be adaptable to explore non-HIF substrates of PHDs and potentially to high-throughput assays.
Topics & Concepts
BiochemistryChemistryColorimetryMicrochemistryChromatographyCancer, Hypoxia, and MetabolismMetabolomics and Mass Spectrometry StudiesMitochondrial Function and Pathology