Bacterial Communities Associated With Abnormal Nugent Score in Postmenopausal Versus Premenopausal Women
Caroline M. Mitchell, Sujatha Srinivasan, Nanxun Ma, Susan D. Reed, Michael C. Wu, Noah G. Hoffman, D. J. Valint, Sean Proll, Tina L. Fiedler, Kathy Agnew, Katherine A. Guthrie, David N. Fredricks
Abstract
The Nugent score is the reference standard for bacterial vaginosis (BV) diagnosis but has not been validated in postmenopausal women. We compared relative abundances from 16S ribosomal RNA gene sequencing of vaginal microbiota with Nugent score in cohorts of premenopausal (n = 220) and postmenopausal (n = 144) women. In premenopausal women, 33 taxa were significantly correlated with Nugent score, including the classic BV-associated taxa Gardnerella, Atopobium, Sneathia, Megasphaera, and Prevotella. In postmenopausal women, 11 taxa were significantly associated with Nugent score, including Prevotella but no other BV-associated genera. High Nugent scores should not be used to infer BV in postmenopausal women.