Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9
Gaku Akimoto, Arianna P Fernandes, Jeffrey W. Bode
Abstract
for the facile preparation of monoubiquitylated proteins.
Topics & Concepts
UbiquitinThioesterFusion proteinEnzyme kineticsEnzymeProtein engineeringBiochemistryUbiquitin-conjugating enzymeChemistryPeptideEscherichia coliUbiquitinsIn vitroBiologyActive siteRecombinant DNAUbiquitin ligaseGeneUbiquitin and proteasome pathwaysGlycosylation and Glycoproteins ResearchPeptidase Inhibition and Analysis