Development of a Glycerol-Inducible Expression System for High-Yield Heterologous Protein Production in Bacillus subtilis
Laichuang Han, Qiaoqing Chen, Jie Luo, Wenjing Cui, Zhemin Zhou
Abstract
In this study, a GIES was constructed in B. subtilis by employing the antiterminator protein GlpP and the GlpP-regulated promoter P glpD . Based on the sequential utilization of glucose and glycerol by B. subtilis , the GIES was able to express genes in an autoinducible manner.
Topics & Concepts
Bacillus subtilisHeterologousGlycerolBiologyGeneHeterologous expressionYield (engineering)BiotechnologyComputational biologyBiochemistryGeneticsRecombinant DNABacteriaMaterials scienceMetallurgyBacterial Genetics and BiotechnologyBacteriophages and microbial interactionsViral Infectious Diseases and Gene Expression in Insects