Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes
Anne K. Engmann, John J. Hatch, Prakruti Nanda, Priya Veeraraghavan, Abdulkadir Özkan, Alexandros Poulopoulos, Alexander J. Murphy, Jeffrey D. Macklis
Topics & Concepts
BiologyProteomeCell biologyPopulationCell sortingRNA extractionRNATranscriptomeComputational biologyMolecular biologyFlow cytometryBiochemistryGene expressionGeneDemographySociologyUbiquitin and proteasome pathwaysRNA Research and SplicingATP Synthase and ATPases Research