Rational design of a turn-on near-infrared fluorescence probe for the highly sensitive and selective monitoring of carboxylesterase 2 in living systems
Zhixin Chen, Jiaying Yu, Kai Sun, Jia Song, Lucheng Chen, Yong Jiang, Zhifei Wang
Abstract
∼ 850 nm), which led to a notable red-shift in the fluorescence (∼175 nm) spectrum. Confocal imaging of cells and live mice demonstrated that the fluorescent signal of this probe was related to the real activities of CES2 in cancer cells. All these results will powerfully promote the screening of CES2 regulators and the analysis of CES2-related physiological and pathological processes.
Topics & Concepts
CarboxylesteraseFluorescenceChemistryRational designTurn (biochemistry)BiophysicsNanotechnologyEnzymeBiochemistryMaterials scienceBiologyOpticsPhysicsCholinesterase and Neurodegenerative DiseasesElectrochemical sensors and biosensorsMolecular Sensors and Ion Detection