RecT Recombinase Expression Enables Efficient Gene Editing in <i>Enterococcus</i> spp.
Victor Chen, Matthew E. Griffin, Pascal Maguin, Andrew Varble, Howard C. Hang
Abstract
Enterococcus faecium is widely recognized as an emerging public health threat with the rise of drug resistance and nosocomial infections. Nevertheless, commensal Enterococcus strains possess beneficial health functions in mammals to upregulate host immunity and prevent microbial infections.
Topics & Concepts
Enterococcus faeciumBiologyRecombineeringCRISPRRecombinaseGeneticsGeneCas9Genome editingMicrobiologyComputational biologyGenomeAntibioticsRecombinationCRISPR and Genetic EngineeringBacterial Genetics and BiotechnologyRNA and protein synthesis mechanisms