Resistance Risk and Resistance-Related Point Mutation in SdhB and SdhC<sub>1</sub> of Cyclobutrifluram in <i>Fusarium pseudograminearum</i>
Yiwen Li, Yidong Tang, Ziwei Xue, Yan Wang, Yifei Shi, Xuheng Gao, Li Xiong, Guixiang Li, Feng Li, Liang Lu, Jianqiang Miao, Xili Liu
Abstract
Cyclobutrifluram is a novel succinate dehydrogenase inhibitor (SDHI) developed by Syngenta and helps to inhibit Fusarium pseudograminearum . Here, the potential for cyclobutrifluram resistance in F. pseudograminearum and the resistance mechanism involved were evaluated. Baseline sensitivity of F. pseudograminearum to cyclobutrifluram was determined with a mean EC 50 value of 0.0248 μg/mL. Fungicide adaption generated five resistant mutants, which possess a comparable or a slightly impaired fitness compared to corresponding parental isolates. This indicates that the resistance risk of F. pseudograminearum to cyclobutrifluram might be moderate. Cyclobutrifluram-resistant isolates also demonstrated resistance to pydiflumetofen but sensitivity to carbendazim, phenamacril, tebuconazole, fludioxonil, or pyraclostrobin. Additionally, point mutations H248Y in FpSdhB and A83V or R86K in FpSdhC 1 were found in cyclobutrifluram-resistant F. pseudograminearum mutants. Molecular docking and overexpression transformation assay revealed that FpSdhB H248Y and FpSdhC 1 A83V or FpSdhC 1 R86K confer the resistance of F. pseudograminearum to cyclobutrifluram.