A GoldenBraid cloning system for synthetic biology in social amoebae
Peter Kundert, Alejandro Sarrion‐Perdigones, Yezabel Gonzalez, Mariko Katoh‐Kurasawa, Shigenori Hirose, Peter Lehmann, Koen J. T. Venken, Gad Shaulsky
Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
Topics & Concepts
BiologyCloning (programming)Synthetic biologyComputational biologyGeneticsEvolutionary biologyProgramming languageComputer scienceProtist diversity and phylogenyCellular Mechanics and InteractionsLegionella and Acanthamoeba research