New strain of <i>Pediococcus pentosaceus</i> alleviates ethanol-induced liver injury by modulating the gut microbiota and short-chain fatty acid metabolism
Xianwan Jiang, Yating Li, Jianzhong Ye, Longxian Lv, Li-Ya Yang, Xiaoyuan Bian, Wenrui Wu, Jingjing Wu, Ding Shi, Qing Wang, Daiqiong Fang, Kaicen Wang, Qiangqiang Wang, Yanmeng Lu, Jiaojiao Xie, Lanjuan Li
Abstract
BACKGROUND: exerts a protective effect on ALD and to elucidate the potential mechanism. AIM: on ethanol-induced liver injury in mice. METHODS: . One dose of isocaloric maltose dextrin or ethanol was administered by oral gavage on day 11, and the mice were sacrificed nine hours later. Blood and tissue samples (liver and gut) were harvested to evaluate gut barrier function and liver injury-related parameters. Fresh cecal contents were collected, gas chromatography-mass spectrometry was used to measure short-chain fatty acid (SCFA) concentrations, and the microbiota composition was analyzed using 16S rRNA gene sequencing. RESULTS: and increased propionic acid and butyric acid production by modifying SCFA-producing bacteria. Furthermore, the levels of the tight junction protein ZO-1, mucin proteins (mucin [MUC]-1, MUC-2 and MUC-4) and the antimicrobial peptide Reg3β were increased after probiotic supplementation. CONCLUSION: alleviated ethanol-induced liver injury by reversing gut microbiota dysbiosis, regulating intestinal SCFA metabolism, improving intestinal barrier function, and reducing circulating levels of endotoxin and proinflammatory cytokines and chemokines. Thus, this strain is a potential probiotic treatment for ALD.