DNA Repair Factor Poly(ADP-Ribose) Polymerase 1 Is a Proviral Factor in Hepatitis B Virus Covalently Closed Circular DNA Formation
Yingshan Chen, Yongxuan Yao, Kaitao Zhao, Canyu Liu, Yifei Yuan, Hao Sun, Dan Huang, Yi Zheng, Yuan Zhou, Jizheng Chen, Yun Wang, Chunchen Wu, Bixiang Zhang, Yujuan Guan, Feng Li, Rongjuan Pei, Xinwen Chen
Abstract
cccDNA formation. HBV infection caused PARylation through PARP1 in Huh7-NTCP cells, primary human hepatocytes, and human-liver chimeric mice. We found that PARP1 could directly bind to the rcDNA lesions and was activated, PARylating other DNA repair proteins. We address the importance of PARP1-mediated PARylation in HBV cccDNA formation, which is a potential therapeutic target for chronic hepatitis B.
Topics & Concepts
cccDNABiologyPARP1Hepatitis B virusPolymeraseVirologyDNA repairDuck hepatitis B virusMolecular biologyDNA polymeraseDNAPoly ADP ribose polymeraseHepadnaviridaeVirusGeneticsHBsAgPARP inhibition in cancer therapyHepatitis B Virus StudiesDNA Repair Mechanisms