CASMART, a one-step CRISPR Cas12a-mediated isothermal amplification for rapid and high-resolution digital detection of rare mutant alleles
Chanqiong Zhang, Zhengyi Cai, Zihao Zhou, Mei Li, Weilong Hong, Wenxian Zhou, Dian‐Jun Yu, Panpan Wei, Jialin He, Yujuan Wang, Chongan Huang, Xiaobing Wang, Jinyu Wu
Topics & Concepts
AlleleDigital polymerase chain reactionMultiplexGenotypingCRISPRGeneticsBiologyMutantCOLD-PCRAllele frequencyComputational biologyGenotypeMolecular biologyPolymerase chain reactionPoint mutationGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesInnovative Microfluidic and Catalytic Techniques Innovation