Regulatory encoding of quantitative variation in spatial activity of a <i>Drosophila</i> enhancer
Yann Le Poul, Yaqun Xin, Liucong Ling, Bettina Mühling, Rita Jaenichen, David Hörl, David M. Bunk, Hartmann Harz, Heinrich Leonhardt, Yingfei Wang, E. V. Osipova, Mariam Museridze, Deepak Dharmadhikari, Eamonn Murphy, Remo Rohs, Stephan Preibisch, Benjamin Prud’homme, Nicolas Gompel
Abstract
wings, and tested their effect in a reporter assay. Moreover, we developed an analytic framework based on the comprehensive quantification of spatial reporter activity. We show that the quantitative enhancer activity results from densely packed regulatory information along the sequence, and that a complex interplay between activators and multiple tiers of repressors carves the spatial pattern. Our results shed light on how an enhancer reads and integrates trans-regulatory landscape information to encode a spatial quantitative pattern.