Detecting Single Molecule Deoxyribonucleic Acid in a Cell Using a Three‐Dimensionally Integrated Nanopore
Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Takashi Washio, Yoshinobu Baba, Tomoji Kawai
Abstract
Abstract Amplification‐free genome analysis can revolutionize biology and medicine by uncovering genetic variations among individuals. Here, the authors report on a 3D‐integrated nanopore for electrolysis to in situ detection of single‐molecule DNA in a cell by ionic current measurements. It consists of a SiO 2 multipore sheet and a SiN x nanopore membrane stacked vertically on a Si wafer. Single cell lysis is demonstrated by 10 6 V m −1 ‐level electrostatic field focused at the multinanopore. The intracellular molecules are then directly detected as they move through a sensing zone, wherein the authors find telegraphic current signatures reflecting folding degrees of freedom of the millimeter‐long polynucleotides threaded through the SiN x nanopore. The present device concept may enable on‐chip single‐molecule sequencing to multi‐omics analyses at a single‐cell level.