Interrogating surface <i>versus</i> intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates
Pascal Poc, Vanessa A. Gutzeit, Julia Ast, Joon Lee, Ben Jones, Elisa D’Este, Bettina Mathes, Martin Lehmann, David J. Hodson, Joshua Levitz, Johannes Broichhagen
Abstract
Impermeable SNAP-tag substrates allow exclusive labelling of receptors on the cell membrane for nanoscopy, SiMPull and <italic>in vivo</italic> use.
Topics & Concepts
IntracellularSnapTransmembrane proteinReceptorBiophysicsCell membraneCellChemistryCell surface receptorCell biologyBiologyBiochemistryComputer scienceComputer graphics (images)Receptor Mechanisms and SignalingMonoclonal and Polyclonal Antibodies ResearchLipid Membrane Structure and Behavior