Multiple electron transfer pathways of tungsten-containing formate dehydrogenase in direct electron transfer-type bioelectrocatalysis
Tatsushi Yoshikawa, Fumiaki Makino, Tomoko Miyata, Yohei Suzuki, Hideaki Tanaka, Keiichi Namba, Kenji Kano, Keisei Sowa, Yuki Kitazumi, Osamu Shirai
Abstract
)/NADH redox couples-was investigated using structural biology and bioelectrochemistry. FoDH1 is reported to be an enzyme that can realize "direct electron transfer (DET)-type bioelectrocatalysis." However, its 3-D structure, electrode-active sites, and electron transfer (ET) pathways remain unclear. The ET pathways were investigated using structural information, electrostatic interactions between the electrode and the enzyme, and the differences in the substrates. Two electrode-active sites and multiple ET pathways in FoDH1 were discovered.
Topics & Concepts
Formate dehydrogenaseElectron transferChemistryFormateBioelectrochemistryRedoxNAD+ kinaseElectrodePhotochemistryEnzymeElectrochemistryInorganic chemistryBiochemistryCatalysisPhysical chemistryCO2 Reduction Techniques and CatalystsElectrochemical sensors and biosensorsMicrobial Fuel Cells and Bioremediation