Dendritic peptide-conjugated polymeric nanovectors for non-toxic delivery of plasmid DNA and enhanced non-viral transfection of immune cells
Sijia Yi, Sunyoung Kim, Michael P. Vincent, Simseok A. Yuk, Sharan Bobbala, Fanfan Du, Evan A. Scott
Abstract
-PPS-ss-DP (PPDP). A library of self-assembling PPDP polymers was synthesized and screened to identify optimal constructs capable of transfecting macrophages with small (pCMV-DsRed, 4.6 kb) and large (pL-CRISPR.EFS.tRFP, 11.7 kb) plasmids. The optimized PPDP construct transfected macrophages, fibroblasts, dendritic cells, and T cells more efficiently and with less toxicity than a commercial Lipo2K reagent, regardless of pDNA size and under standard culture conditions in the presence of serum. The PPDP technology described herein is a stimuli-responsive polymeric nanovector that can be leveraged to meet diverse challenges in gene delivery.
Topics & Concepts
TransfectionGene deliveryPlasmidMolecular biologyChemistryDNAGenetic enhancementCRISPRGeneBiologyBiochemistryRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesVirus-based gene therapy research