The nanotopography of SiO<sub>2</sub>particles impacts the selectivity and 3D fold of bound allergens
Robert Mills-Goodlet, Litty Johnson, Isabel J. Hoppe, Christof Regl, Mark Geppert, Milena Schenck, Sara Huber, Michael Hauser, Fátima Ferreira, Nicola Hüsing, Christian G. Huber, Hans Brandstetter, Albert Duschl, Martin Himly
Abstract
nanoparticles influenced the 3D fold of the protein, resulting in at least partial unfolding. Consequently, this conformational change influenced the allergenic response, as observed by mediator release assays employing the sera of patients and immune effector cells. For an in-depth understanding of the bio-nano interactions, the properties of the particles need to be considered not only regarding the identity and morphology of the material, but also their nanotopography, given that porosity may greatly influence the structure, and hence the biological behaviour of the bound proteins. Thus, thorough structural investigations upon the formation of protein corona are important when considering immunological outcomes, as particle binding can influence the allergenic response elicited by the bound allergen.