A functionalized magnetic nanoparticle regulated CRISPR-Cas12a sensor for the ultrasensitive detection of alpha-fetoprotein
Ya Liu, Yan Chen, Yue Zhang, Qi Zhong, Xiaoli Zhu, Qi Wu
Abstract
. In addition, we have successfully applied this biosensor to detect AFP in clinical samples from patients with hepatoblastoma, with greater sensitivity relative to ELISA. Our proposed method showed great potential application in clinical diagnosis and pharmaceutical-related fields with the properties of high sensitivity, low cost and high selectivity.
Topics & Concepts
HepatoblastomaAptamerNucleic acidDetection limitAlpha-fetoproteinCRISPRChemistryBiosensorMagnetic nanoparticlesLinear rangeMolecular biologyNanoparticleChromatographyBiochemistryNanotechnologyCancer researchBiologyMaterials scienceHepatocellular carcinomaGeneMedicineRadiologyCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRetinal Development and Disorders