Silencing long non-coding RNA NEAT1 attenuates rheumatoid arthritis via the MAPK/ERK signalling pathway by downregulating microRNA-129 and microRNA-204
Jie Chen, Xiao Luo, Mao Liu, Lihui Peng, Zixia Zhao, Chengsong He, Yue He
Abstract
ACR: American College of Rheumatology; ELISA: Enzyme-linked immunosorbent assay; ERK: extracellular signal-regulated kinase; FLS: fibroblast-like synoviocyte; GADPH: glyceraldehyde-3-phosphate dehydrogenase; HRP: horseradish peroxidase; IFA: Incomplete Freund's Adjuvant; lncRNAs: long noncoding RNAs; MSP: Methylation-specific PCR; NC: negative control; NEAT1: nuclear paraspeckle assembly transcript 1; OD: optical density; RA: rheumatoid arthritis; RIPA: Radio Immunoprecipitation Assay; RLU: relative light units; RT-qPCR: reverse transcription quantitative polymerase chain reaction; UTR: untranslated region.
Topics & Concepts
Gene silencingmicroRNAMAPK/ERK pathwayBiologyRNA interferenceCancer researchKinaseRegulation of gene expressionDownregulation and upregulationLong non-coding RNACell biologyMolecular biologyRNAGeneGeneticsCancer-related molecular mechanisms researchCircular RNAs in diseasesMicroRNA in disease regulation