Role of the ESCRT Pathway in Laccase Trafficking and Virulence of Cryptococcus neoformans
Yoon‐Dong Park, Shu Hui Chen, Emma Camacho, Arturo Casadevall, Peter R. Williamson
Abstract
The endosomal sorting complex required for transport (ESCRT) plays a crucial role in the transportation and degradation of proteins. We determined that Vps27, a key protein of the ESCRT-0 complex, is required for the transport of the virulence factor laccase to the cell wall in Cryptococcus neoformans . Laccase activity was perturbed, as was melanin production, in vps27 Δ strains. In the absence of VPS27 , there was an accumulation of multivesicular bodies with vacuolar fragmentation and mistargeting of the vacuolar carboxypeptidase CPY/Prc1, resulting in an extracellular localization.
Topics & Concepts
ESCRTCryptococcus neoformansEndosomeBiologyVirulenceVacuolar protein sortingVirulence factorCell biologyExtracellularMicrobiologyFungal proteinTransport proteinIntracellularYeastBiochemistrySaccharomyces cerevisiaeGeneFungal Infections and StudiesPhytoplasmas and Hemiptera pathogensAntifungal resistance and susceptibility