Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate
Hong Yao, Jing Cheng, Aijuan Li, Runhao Yu, Wenbo Zhao, Shangshang Qin, Xiang‐Dang Du
Abstract
Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both blaIMP-26 and tet(A) variant in a clinical Klebsiella pneumoniae KP-1572. MIC results showed that K. pneumonia KP-1572 was resistant to a wide range of antimicrobials. The blaIMP-26 and tet(A) variant were located on an identical plasmid, which was indicated by S1-PFGE and southern blotting hybridization and can be successfully transferred by electroporation. Whole plasmid sequencing and analysis revealed that a 142,993 bp plasmid in size, designated pIMP1572, contains an IncFIIk backbone and variable region harboring blaIMP-26 and tet(A) variant. The plasmid pIMP1572 was apparently originated from a tet(A)-carrying IncFIIk plasmid but with a length of 6,216 bp deletion and multiple drug resistance region (MDRR) insertion of 25,259 bp. The plasmid pIMP1572 in the present study represents the first report of IncFIIk plasmid co-carrying blaIMP and tet(A) variant, which should be monitored.