Protein Design: From the Aspect of Water Solubility and Stability
Rui Qing, Shilei Hao, Eva Smorodina, David Jin, Arthur O. Zalevsky, Shuguang Zhang
Abstract
transmembrane protein design. Traditional strategies to enhance protein solubility and structural stability are introduced. The designs of stable protein complexes and high-order assemblies are covered. Computational methodologies behind these endeavors, including structure prediction programs, machine learning algorithms, and specialty software dedicated to the evaluation of protein solubility and aggregation, are discussed. The findings and opportunities for Cryo-EM are presented. This review provides an overview of significant progress and prospects in accurate protein design for solubility and stability.
Topics & Concepts
SolubilityStability (learning theory)Protein stabilityProtein designChemistryTransmembrane proteinComputer scienceBiochemical engineeringProtein structureMachine learningEngineeringBiochemistryOrganic chemistryReceptorProtein Structure and DynamicsEnzyme Structure and FunctionRNA and protein synthesis mechanisms