Biocatalytic reductive amination as a route to isotopically labelled amino acids suitable for analysis of large proteins by NMR
Jack S. Rowbotham, Jake H. Nicholson, Miguel A. Ramirez, Kouji Urata, Peter M.T. Todd, Gogulan Karunanithy, Lars Lauterbach, Holly A. Reeve, Andrew J. Baldwin, Kylie A. Vincent
Abstract
C]-alanine and integrated it into a large (>400 kDa) heat-shock protein oligomer, which was subsequently analysable by methyl-TROSY techniques, revealing new structural information.
Topics & Concepts
Reductive aminationAminationAmino acidChemistryCombinatorial chemistryOrganic chemistryBiochemistryCatalysisChemical Reactions and IsotopesAmino Acid Enzymes and MetabolismPharmacogenetics and Drug Metabolism