Whole Exome Sequencing of <i>SMO</i>, <i>BRAF</i>, <i>PTCH1</i> and <i>GNAS</i> in Odontogenic Diseases
Michiko Shimura, Koh‐ichi Nakashiro, Yuta Sawatani, Tomonori Hasegawa, RYOTA KAMIMURA, Sayaka Izumi, Yuske Komiyama, Chonji Fukumoto, Shuma Yagisawa, Erika Yaguchi, Masayo Hitomi-Koide, Toshiki Hyodo, Daisuke Uchida, Hitoshi Kawamata
Abstract
BACKGROUND/AIM: Odontogenic diseases are diagnosed based on clinical course, imaging, and histopathology. However, a definitive diagnosis is not always possible. PATIENTS AND METHODS: We analyzed whole exons of SMO, BRAF, PTCH1 and GNAS using next-generation sequencing (NGS) in 18 patients. RESULTS: Of the 6 patients with ameloblastoma, 2 patients had the same missense mutation in BRAF, and 1 patient with peripheral ameloblastoma had a missense mutation in PTCH1. Of the 7 patients with odontogenic keratocyst, 4 patients had a missense mutation in PTCH1, 2 patients had missense mutations in BRAF, and 1 patient had a missense mutation in SMO. The patient with odontoma had missense mutations in SMO, BRAF and PTCH1. One patient with cement-osseous dysplasia had missense mutations in SMO and PTCH1. The patient with adenomatoid odontogenic tumor had missense mutations in SMO. CONCLUSION: Whole exome sequencing of the above genes by NGS would be useful for the differential diagnosis of odontogenic diseases.