Alternative Assay Reagents for UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB Module
Felix Kaspar, Charity S. G. Ganskow, Lea Eilert, Philipp Klahn, Anett Schallmey
Abstract
This report advises against the use of 5-iodoridine or 5-ethynyluridine as alternative assay reagents in the PUB module, primarily due to their lack of an isosbestic point of phosphorolysis under moderately alkaline conditions.
Topics & Concepts
ChemistryPhosphorolysisReagentPhosphateNuclear chemistryChromatographyOrganic chemistryPurineEnzymePurine nucleoside phosphorylaseMass Spectrometry Techniques and ApplicationsMolecular Sensors and Ion DetectionAdvanced Chemical Sensor Technologies