Designer tetrahedral DNA framework-based microfluidic technology for multivalent capture and release of circulating tumor cells
Chenguang Wang, Yi Xu, Shuainan Li, Yi Zhou, Qiuling Qian, Yifan Liu, Xianqiang Mi
Abstract
using our T-μFS in the whole blood. The release efficiency of the MCF-7 cells was 96.2% and the MCF-7 cell viability after release was 94.6% using our T-μFS in PBS buffer. Reculture and RT-qPCR studies showed that there was almost no damage by the capture and release treatment for the MCF-7 cells viability. These results revealed that our T-μFS could be developed as an integrated and automatic technical platform with great performance for multivalent capture and release of CTCs and have a wide application prospect for tumor liquid biopsy.
Topics & Concepts
Circulating tumor cellAptamerMicrofluidicsLiquid biopsyViability assayBiomarkerNanotechnologyChemistryDNACancer researchBiophysicsMolecular biologyCancerCellMaterials scienceBiologyBiochemistryMedicineInternal medicineMetastasisAdvanced biosensing and bioanalysis techniquesCancer Genomics and DiagnosticsMicrofluidic and Bio-sensing Technologies