Synthesis of cyclodextrin‐based temperature/enzyme‐responsive nanoparticles and application in antitumor drug delivery
Jinkui Teng, Lulu Yue, Bilian Li, Jianmei Yang, Cuiting Yang, Tong Yang, Xiangye Zhi, Xiaoqing Liu, Yan Zhao, Jin Zhang
Abstract
Nanocarrier systems including the lipid-based, silica nanoparticle (NP)-based, chitosan-based, cyclodextrin (CD)-based, and so forth were prepared based on the various stimulus containing redox, pH, temperature, enzymes, etc. The nanocarriers with dual stimuli-responsive properties can meet further practical and clinical applications due to their highly accurate and controllable response to the lesion site, good drug release effect, and weak side effects. Here, a temperature/enzyme-responsive nanocarrier was prepared based on seven-[6-(diaminodipropylamine)-6-deoxy] -β-CD (SA-β-CD) and lauroyl choline chloride (LCC). The SA-β-CD/LCC NPs showed trapping and releasing properties for the antitumor drug celastrol (CSL) in the synergy of the temperature/enzyme stimuli. Notably, the release rate of CSL-trapped NPs reached 94%, which is a relatively high value in numerous drug delivery systems using CSL as a drug model. Furthermore, cytotoxicity and apoptosis tests suggested that CSL-trapped NPs exhibited similar activity to intact CSL on five tumor cells. The dual response nanocarriers may pave a possible way for effectively targeted releasing anti-cancer drugs.