Mutations in the 3'-PPT Lead to HIV-1 Replication without Integration
Clémence Richetta, Frédéric Subra, Isabelle Malet, Hervé Leh, Charlotte Charpentier, Angela Corona, Gilles Collin, Diane Descamps, Eric Deprez, Vincent Parissi, Vincent Cálvez, Enzo Tramontano, Anne‐Geneviève Marcelin, Olivier Delelis
Abstract
Our work highlights the role of HIV-1 unintegrated viral DNA in viral replication. A virus, resistant to strand-transfer inhibitors, has been selected in vitro . This virus highlights a mutation in the 3'PPT region and not in the integrase gene. This mutation modifies the reverse transcription step leading to the accumulation of 1-LTR circles and not the linear DNA. This accumulation of 1-LTR circles leads to viral replication without integration of the viral genome.
Topics & Concepts
BiologyIntegraseViral replicationVirologyMutationGenomeReverse transcriptaseVirusIntegrase inhibitorGeneGeneticsDNAHuman immunodeficiency virus (HIV)Viral loadPolymerase chain reactionAntiretroviral therapyHIV Research and TreatmentHIV/AIDS drug development and treatmentCytomegalovirus and herpesvirus research