Radioiodinated esomeprazole as a model for peptic ulcer localization
M. H. Sanad, H. M. Eyssa, Nermien M. Gomaa, F. A. Marzook, Sabry A. Bassem
Abstract
Abstract This work focuses on tracking stomach ulcer localized in mice. High labeling yield and radiochemical purity were achieved for the formation of a [ 125 I]esomeprazole radiotracer at optimum conditions of oxidizing agent content (chloramines-T (Ch-T), 100 μg), substrate amount (Esom, 100 μg), pH of reaction mixture (6), reaction time (30 min) and temperature (37 °C), using radioactive iodine-125 (200–450 MBq). The radiolabeled compound, [ 125 I]esom, was stable in serum and saline solution during 24 h. Esom is acting as a histamine-2-receptor antagonist (H 2 RA). Biodistribution studies were carried in normal and ulcerated mice. High uptake of 78.12 ± 0.80% injected dose/g organ (ID/g) observed in ulcerated mice confirmed the suitability of [ 125 I]esomeprazole as a novel radiotracer for stomach imaging.